• IN VITRO ANTIOXIDANT ACTIVITIES OF AVENA SATIVA (OATS) ALCOHOLIC SEED EXTRACTS

Abstract

The research envisaged was carried out to explore the antioxidant potential of alcoholic seed extract of Avena Sativa (Oats) invitro. The seeds ofAvenasativawere collected and air dried seed sample rinsed with water and extracted with ethanol in a soxhlet apparatus. The suspension of extract was prepared freshly in normal saline with the help of 0.5% w/v carboxymethylcellulose (CMC). Varying concentration of extract (10, 25, 50 and 100 µg/ml) was tested for invitro antioxidant properties. Antioxidant activity was determined by DPPH assay, reducing power ability, hydrogen peroxide scavenging assay and hydroxyl radical (OH^-) scavenging activity. The ethanolic seed extract of Oats demonstrated antioxidant potential dose dependently with best activity at100 µg/ml. Based on the outcomes of the present research, anti-oxidative potential could be attributed for traditionally claimed potencies of seeds of Avena Sativa (Oats). However, further studies to be carried out on animal models using their biological tissues before exploiting for its clinical benefits.